Antifilarial effects of polyphenol rich ethanolic extract from the leaves of Azadirachta indica through molecular and biochemical approaches describing reactive oxygen species (ROS) mediated apoptosis of Setaria cervi

Article ID	Journal	Published Year	Pages	File Type
4371146	Experimental Parasitology	2014	18 Pages	PDF

Abstract

- S. cervi is widely used as a model to screen antifilarials.
- Polyphenol rich EtOH extract of A. indica leaves were optimized by RSM.
- EEA was tested against mf and adults of S. cervi cultured in vitro.
- Morphological, molecular and biochemical alterations were examined.
- Apoptosis and ROS up regulation are the probable cause of effectiveness.

Keywords

GST egg laying defective apoptotic protease activating factor APAF microfilariae EEA EGL EtBr NBT Bcl-2 RT-PCR TPC GPX GSH EtOH PVDF CED PBS p53 FBS Azadirachta indica DMSO MTT ROS Setaria cervi nitroblue tetrazolium opd Ethanol ethidium bromide ortho-phenylenediamine analysis of variance ANOVA Terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL Apoptosis Dimethyl sulfoxide Culture media Response surface methodology RSM SOD foetal bovine serum Superoxide dismutase Phosphate buffered saline B-cell lymphoma 2 reverse transcriptase polymerase chain reaction protein 53 Glutathione glutathione-S-transferase glutathione peroxidase Reactive oxygen species (ROS)Reactive oxygen species