Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4371711 | Experimental Parasitology | 2010 | 6 Pages |
Abstract
A 500Â bp fragment encoding an isoform of cysteine proteinase from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24Â kDa, rLacys24. In Western blots of L. (L.) amazonensis extracts, antibodies directed to rLacys24 recognized a cysteine proteinase isoform of 30Â kDa. Analysis by fluorescence-activated cell sorter showed a significantly higher expression of CD8+ lymphocytes in animals immunized with rLacys24 plus CFA, whereas a low expression of CD4+ lymphocytes was observed in these animals. The cytotoxicity of lymphocytes isolated from mice immunized with rLacys24 plus CFA on L. (L.) amazonensis-infected macrophages was significantly higher than that observed in the presence of lymphocytes from control animals. Immunization of BALB/c mice with rLacys24 plus CFA resulted in a low but significant decrease of foot lesions after challenge with L. (L.) amazonensis compared to those exhibited by control mice.
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Life Sciences
Immunology and Microbiology
Parasitology
Authors
Carlos Eduardo Cardoso Fedeli, Josie Haydée Lima Ferreira, Juliana Sekeres Mussalem, Ieda Maria Longo-Maugéri, Luciana Girotto Gentil, Márcia Regina Machado dos Santos, Simone Katz, Clara Lúcia Barbiéri,