Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4371733 | Experimental Parasitology | 2009 | 7 Pages |
Abstract
Effects of essential oil of Allium sativum (garlic) and Piper longum (Indian long pepper) were evaluated on muscular activity of whole Fasciola gigantica and its strip preparation. The whole flukes and longitudinal strip preparations of the flukes were isometrically mounted to record the spontaneous muscular activity (SMA) and to evaluate effects of cumulative doses (0.1, 0.3, 1.0 and 3.0Â mg/ml) of the plant essential oils. Whole flukes and the strip preparations exhibited continuous SMA without any significant difference in its baseline tension, frequency and amplitude for 2Â h. Essential oil of A. sativum produced significant reduction in the frequency and the amplitude of the SMA of whole fluke at 1 and 3Â mg/ml concentrations. It caused complete paralysis of the fluke after 15Â min of administration of 3Â mg/ml concentration. Similar to whole fluke, essential oil of A. sativum (3Â mg/ml) also produced flaccid paralysis in the strip preparations of the flukes. Essential oil of P. longum firstly induced marked excitatory effect and then there was flaccid paralysis of the whole fluke following 15Â min exposure at 3Â mg/ml concentration. Complete flaccid paralysis of the strip preparation was also ensued after 15Â min of administration of 3Â mg/ml concentration of P. longum. In both the essential oils, the whole fluke and strip preparations did not recover from paralysis following 2-3 washes. In conclusion, the observations demonstrated irreversible paralytic effect of essential oils of A. sativum and P. longum on F. gigantica in vitro which might possibly help to developing herbal-based anthelmintic.
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
Thakur Uttam Singh, Dinesh Kumar, Surendra Kumar Tandan, Santosh Kumar Mishra,