Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4372066 | Experimental Parasitology | 2006 | 10 Pages |
Abstract
Study of the parasite mosquito stages of Plasmodium and its use in the production of sporozoite vaccines against malaria has been hampered by the technical difficulties of in vitro development. Here, we show the complete axenic development of the parasite mosquito stages of Plasmodium yoelii. While we demonstrate that matrigel is not required for parasite development, soluble factors produced and secreted by Drosophila melanogaster S2 cells appear to be crucial for the ookinete to oocyst transition. Parasites cultured axenically are both morphologically and biologically similar to mosquito-derived ookinetes, oocysts, and sporozoites. Axenically derived sporozoites were capable of producing an infection in mice as determined by RT-PCR; however, the parasitemia was significantly much less than that produced by mosquito-derived sporozoites. Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations.
Keywords
RT-PCRrRNADrosophila melanogaster S2 cellsP. yoeliiFDAAxenicmsp1Plasmodium yoeliiEMEMEtBrRBCkiloDaltonkDaHRPPBSmAbcDNADNARibosomal RNAMonoclonal antibodydeoxyribonucleic acidcomplementary deoxyribonucleic acidRNAribonucleic acidELISAEnzyme-linked immunosorbent assayroom-temperatureFood and Drug AdministrationS2 cellsMalariaPhosphate-buffered salinewild-typeReverse transcriptase-polymerase chain reactionHorseradish peroxidaseCircumsporozoite proteinMerozoite surface protein 1red blood cell
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
Johanna M. Porter-Kelley, Rhoel R. Dinglasan, Uzma Alam, George A. Ndeta, John B. Jr., Abdu F. Azad,