| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4372070 | Experimental Parasitology | 2006 | 4 Pages |
Abstract
We report the use of representational difference analysis to identify genes that have up-regulated expression in the amastigote life-cycle stage of Leishmania (Viannia) panamensis. This simultaneous process of selection and amplification allowed the cloning of several specific DNA fragments. One of them shows a high percentage of similarity with histone H1 genes from other Trypanosomatids and, as expected, is up-regulated in the amastigote life-cycle stage. The gene is present in two copies that are expressed at different levels in promastigotes and also in amastigotes, which seems to be a consequence of their different 3â² untranslated regions.
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Immunology and Microbiology
Parasitology
Authors
Juan F. Alzate, J. Robinson RamÃrez-Pineda, Victor M. González, Edwin Patiño, Ivan D. Vélez, Antonio Jiménez-Ruiz,