Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4372094 | Experimental Parasitology | 2008 | 10 Pages |
Abstract
Taenia solium cysticercosis is a significant public health problem in endemic countries. The current serodiagnostic techniques are not able to differentiate between infections with viable cysts and infections with degenerated cysts. The objectives of this study were to identify specific novel biomarkers of these different disease stages in the serum of experimentally infected pigs using ProteinChip technology (Bio-Rad) and to validate these biomarkers by analyzing serum samples from naturally infected pigs. In the experimental sample set 30 discriminating biomarkers (p < 0.05) were found, 13 specific for the viable phenotype, 9 specific for the degenerated phenotype and 8 specific for the infected phenotype (either viable or degenerated cysts). Only 3 of these biomarkers were also significant in the field samples; however, the peak profiles were not consistent among the two sample sets. Five biomarkers discovered in the sera from experimentally infected pigs were identified as clusterin, lecithin-cholesterol acyltransferase, vitronectin, haptoglobin and apolipoprotein A-I.
Keywords
IgGApo A-IVWCXSELDI-TOF MSApo A-ILCATIEFIMACDTTSDSHDLEDM3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateCestodem/zS/NApolipoprotein A-IApolipoprotein A-IVimmunoglobulin GWeak cation exchangeisoelectric focusingPigdithiothreitolsodium dodecyl sulfateCysticercosisCoefficient of Variationlecithin-cholesterol acyltransferasehigh density lipoproteinmass to charge ratiosignal to noise ratiobiological markersMolecular weightCHAPSTaenia soliumquality control
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
N. Deckers, P. Dorny, K. Kanobana, J. Vercruysse, A.E. Gonzalez, B. Ward, M. Ndao,