| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4372098 | Experimental Parasitology | 2008 | 4 Pages |
Abstract
A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
Keywords
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Immunology and Microbiology
Parasitology
Authors
Kanasinakatte R. Umesha, Sanath Kumar, Ammini Parvathi, Kunyarat Duenngai, Paiboon Sithithaworn, Indrani Karunasagar, Iddya Karunasagar,