Decolorization of Remazol Brilliant Blue R by New Isolated White Rot Fungus Collected from Tropical Rain Forest in East Kalimantan and its Ligninolytic Enzymes Activity

In this research, decolorization of Remazol Brilliant Blue R (RBBR) was investigated using a new isolated white rot fungus, strain KRUS-G, collected from Mulwarman University Botanical Garden,Samarinda, East Kalimantan, Indonesia. The results showed that strain KRUS-G decolorized RBBR efectively compared to Phanerochaetecrysosporiumand Ceriporiopsissubvermispora. The highest decolorization was obtained at pH 4 with 89% loss of RBBR used. The effective decolorization was also observed in the high concentration of RBBR (1500 ppm).Concentration of RBBR at 500, 1000, and 1500 ppm caused slightly decreasing pattern on mycelia growth. Rapid decolorization was occurred within 4 days of incubation. We suggest that laccase played an important role on the decolorizationeven high activity ofMnP was also detected.