Production of Microbial Oils fermented by Two-step Fermentation with Corn stalk

The corn stalk was first fermented by Trichoderma koningii to produce reducing sugar, then the production was re-fermented by Trichosporon cutaneum to produce microbial oils. The best process conditions were obtained by orthogonal test through measuring the content of reducing sugar and microbial oils in product. The result shows that the reducing sugar content of the corn stalk fermented by Trichoderma koningii under the additive of Trichoderma koningii is 11% at ph6 for 5d was 2.385 mg/ml. The microbial oils content of the corn stalk re-fermented by Trichosporon cutaneum under the additive of Trichosporon cutaneum is 9% at 28°C for 7d was 1.276 g per 100 g fermentation products. The research provides a new way for utilization of corn stalk and also provides a new method for the development of microbial oils.