Effect of some factors on Production of cellulase by Trichoderma reesei HY07

The effects of some factors including ammonium sulphate, inoculum, Tween 80, temperature and pH were assessed for the production of cellulase by Trichoderma reesei HY07 isolated from decaying corn stalk. The results were as follows: the optimal concentrations of Ammonium sulphate, inoculum, Tween 80 for production of carboxymethyl cellulase (CMCase) were 1.5% (w/w), 2.5%(v/w) and 0.1% (v/w) and the optimal temperature for production of CMCase were 30°C The optimal concentrations of ammonium sulphate, inoculum, Tween 80 for production of filter paper activity (FPA) were 0.5%(w/w),1.5%(v/w) and 0.1%(v/w) and the optimal temperature for production of FPA were 30°C.