| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4407726 | Chemosphere | 2016 | 10 Pages |
•Testicular transcriptome of adult male rare minnow was analyzed in response to BPA.•Steroid hormone, BTB, proteolysis, and lipid metabolism related genes were altered.•BPA at 1 μg/L possibly initiate mER.•1 and 15 μg/L BPA inhibited tRNA processing-related genes expression.•15 and 225 μg/L BPA decreased hemostasis and blood coagulation-related gene expression.
Bisphenol A (BPA) is widely spread in the environment. It can cause various reproductive disrupting effects on different organisms, including fish. To investigate the effect of BPA at different concentrations comprehensively, RNA-seq was performed on the testicular mRNA libraries of adult male rare minnow Gobiocypris rarus that exposed to 0, 1, 15 and 225 μg/L BPA for 7 days. Meanwhile, biological indicators and sex steroid hormone levels were investigated. Result showed that (1) BPA at all three concentrations affected the expression of genes related to testicular steroid hormone biosynthesis, blood-testis barrier, proteolysis, and lipid transport and metabolism. (2) BPA at 1 μg/L induced gene expression in renin-angiotensin system pathway and possibly initiate membrane form of estrogen receptor (mER); 1 and 15 μg/L BPA inhibited tRNA processing-related genes expression; 15 and 225 μg/L BPA decreased hemostasis and blood coagulation-related gene expression. The present study indicated that BPA did influence rare minnow testicular gene expressing, and the effect BPA effects varied with concentration.
