| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4410432 | Chemosphere | 2012 | 7 Pages |
Enzymes encoded by genes biodegrading microcystins (MCs) can help reveal the function of genes and biodegradation pathway of MCs. Here the first and important gene (USTB-05-A, 1,008 bp) involved in biodegradation of microcystin-RR (MC-RR) was cloned from Sphingopyxis sp. USTB-05 and firstly expressed in Escherichia coli BL21 (DE3) with an expression vector of pGEX4T-1 successfully. The nucleotide sequences of cloned USTB-05-A possessed 92.5% homology to that of mlA reported in Sphingomonas sp. strain ACM-3962. The deduced amino acid sequences containing the cleavage sites of 26th (alanine) and 27th (leucine) showed 83% identical to that of MlrA. The cell-free extract (CE) of recombinant E. coli BL21 (DE3) containing USTB-05-A had high activity for biodegrading MC-RR. Initial MC-RR of 40 mg L−1 was completely biodegraded under total protein of 350 mg L−1 within 0.25 h. A product derived from MC-RR appeared distinctly with the decrease of MC-RR peak on the profile of HPLC. The product (m/z 1056.5) had molecular weight of 18 higher than that of MC-RR (m/z 1038.7). The findings provided the positive evidences that biodegradation of MC-RR began with the breakage of cyclic MC-RR and then it was converted to linear MC-RR as the first product catalyzed by first enzyme of Sphingopyxis sp. USTB-05.
► Gene USTB-05-A (1,008 bp) was cloned from Sphingopyxis sp. USTB-05 biodegrading MC-RR. ► USTB-05-A was successfully expressed in Escherichia coli BL21(DE3) with expression vector pGEX4T-1. ► The expressed enzyme encoded by USTB-05-A had high activity for biodegrading MC-RR. ► Linear product (m/z 1056.5) had molecular weight of 18 higher than that of MC-RR (m/z 1038.7). ► The first step involved in enzymatic pathway for MC-RR biodegraded by Sphingopyxis sp. USTB-05 was characterized.
