Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4411385 | Chemosphere | 2010 | 8 Pages |
Abstract
Glutathione pathway was specifically studied in rats exposed by inhalation to a range of ethylbenzene vapours (5-2000Â ppm). Urines were collected during exposure (6Â h) and over the 18Â h following the exposure. The potential metabolites coming from either side-chain or ring oxidation were synthesized: 1-, 2-phenylethylmercapturic acids (1-, and 2-PEMA) and 2-, 3- and 4-ethylphenylmercapturic acids (2-, 3-, and 4-EPMA). Their synthesis was fully described and the molecules characterized. Urine samples were analysed using a selective HPLC-fluorescence method. Among the five metabolites, 2-PEMA was never observed in any urine sample. By contrast, 1-PEMA was discovered in its two diastereomeric forms, and it was shown that one of them was mainly present. 2-EPMA, 3-EPMA and 4-EPMA (in the ratio 1:2:6) were also found, and their combined excretion levels were similar to that of 1-PEMA. The atmospheric concentrations and urinary excretions yielded very close correlations which allow us to consider these mercapturic acids as novel ethylbenzene exposure biomarkers.
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Authors
Benoît Cossec, Frédéric Cosnier, Manuella Burgart, Hervé Nunge, Stéphane Grossmann,