| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4419020 | Ecotoxicology and Environmental Safety | 2016 | 6 Pages |
•Environmental concentration of nonylphenol (NP) inhibited molting of the mysids.•Some regulated genes associated with molting disruption in the mysid exposed to NP.•Our findings suggested that these genes might become useful molecular biomarkers.
Nonylphenol (NP) has been classified as an endocrine-disrupting chemical. In this study, we conducted mysid DNA microarray analysis with which has 2240 oligo DNA probes to observe differential gene expressions in mysid crustacean (Americamysis bahia) exposed to 1, 3, 10 and 30 μg/l of NP for 14 days. As a result, we found 31, 27, 39 and 68 genes were differentially expressed in the respective concentrations. Among these genes, the expressions of five particular genes were regulated in a similar manner at all concentrations of the NP exposure. So, we focused on one gene encoding cuticle protein, and another encoding cuticular protein analogous to peritrophins 1-H precursor. These genes were down-regulated by NP exposure in a dose-dependent manner, and it suggested that they were related in a reduction of the number of molting in mysids. Thus, they might become useful molecular biomarker candidates to evaluate molting inhibition in mysids.
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