| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4419261 | Ecotoxicology and Environmental Safety | 2016 | 6 Pages |
•A simple method for selective detection of diclofenac in water and blood was developed•Triazole based supramolecule was synthesized through multi-step organic synthesis•Triazole based supramolecule exhibited enhancement in the absorbance intensity after interaction with diclofenac.•The assay was used for determination of diclofenac in tape water of Karachi and in human blood plasma.
A triazole-based supramolecular chemosensor 6 for the selective spectrophotometric detection of diclofenac in human plasma and tap water was developed. The 6 was synthesized through click approach and characterized via UV–vis spectroscopy, FT-IR, Mass and NMR spectroscopy. The supramolecular interaction of compound 6 with commonly used drugs has been investigated with the help of UV–vis spectral titrations, FT-IR and 1H, 13C NMR spectroscopy. The UV–visible spectral changes upon addition of various commonly used drugs showed that compound 6 is highly selective for diclofenac over other drugs. The supramolecule 6 exhibited a selective enhancement in the absorbance intensity after mixing with diclofenac in human plasma and water samples in the presence of other drugs at pH 6–7, with a linear range of 0.938 (R2=0.938) and a limit of detection 10 µM. The relative standard deviation (RSD) for 100 µM diclofenac was found to be 0.462. The Job's plot analysis revealed that diclofenac bind to compound 6 in 1:1 stoichiometry.
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