Evaluation of the genotoxic potential of reactive black 5 solutions subjected to decolorizing treatments by three fungal strains

The genotoxic potential of solutions of the textile dye “Reactive Black 5” that were subjected to decolorizing treatments with the fungal strains Coriolopsis polyzona MUCL33483, Penicillium sp. MUBA001 and Pycnoporus sp. MUBA002 was tested. The genotoxicity of the solutions was determined by evaluation of micronuclei formation in Vicia faba root cells and calculation of a damage index (MNID). Non-treated Reactive Black 5 solutions (50–1000 ppm) caused a statistically significant increase in micronuclei formation and, by then, in damage index. Solutions of dye treated with C. polyzona MUCL33483 and Pycnoporus sp. MUBA002 showed color loss, probably due to enzymatic breakdown of the colorant, but maintenance or even an increase in genotoxicity. On the other hand, the Penicillium sp. strain MUBA001 caused decolorization of the dye, apparently by adsorption on mycelia, and, for solutions that initially contained 50 ppm of colorant, an elimination of the genotoxicity was observed after three weeks of treatment.

► Reactive Black 5 was subjected to decolorizing treatments by three fungal strains. ► Solutions resulting from the decolorizing treatments were tested for genotoxicity. ► Genotoxicity was tested by evaluating micronuclei formation in Vicia fava cells. ► Two of the strains caused decrease in color intensity but no decrease in genotoxicity. ► A third strain caused decrease in color and genotoxicity probably through adsoption.