| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4420852 | Ecotoxicology and Environmental Safety | 2012 | 5 Pages |
In this study an electroantennogram (EAG) method was developed for use on live daphniids. The EAG response of Daphnia magna and Daphnia pulex to a variety of amino acids was measured. The strongest response measured was elicited by L-arginine and was shown to induce a concentration-dependent response indicating the response is olfactory in nature. Subsequent exposures of D. magna to a low, ecologically-relevant concentration of copper (7.5 μg/L) showed a disruption in EAG function. This study utilizes the development of an EAG method for measuring olfactory acuity of live daphniids and demonstrates that at ecologically-relevant concentrations, the olfactory dysfunction caused by copper can be detected. The EAG technique is a useful tool for investigating the olfactory response of daphniids to odourants at the cellular level and detecting the effects of toxicants on the olfactory acuity of daphniids.
► The electroantennogram (EAG) is a viable method for measuring the olfactory response of live daphniids to amino acids. ► Both Daphnia magna and Daphnia pulex responded greatest to amino acid L-arginine. ► Exposure to copper at an ecologically relevant concentration impaired the chemosensation of D. magna by more than 50 percent. ► The EAG can be used in ecological risk assessment of contaminants in aquatic environments on the olfaction of daphniids.
