| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4422404 | Ecotoxicology and Environmental Safety | 2007 | 6 Pages |
Abstract
Two cytosolic glutathione S-transferase (GST) classes were isolated and characterized from California halibut (Paralichthys californicus) liver. Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as θ and α by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) was determined to be 0.23±0.003 U/mg cytosolic protein.
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Authors
Rachel T. Donham, Andrea D. Luna, Sandra Chang, Dexter Morin, William T. Jewell, Ronald S. Tjeerdema,