| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4423730 | Environment International | 2008 | 8 Pages |
In this study, wild three-spined sticklebacks were sampled every six weeks, between April and October, in a low contaminated stream. For all fish, physiological indexes, such as condition factor, hepato-, gonado- and nephro-somatic index were calculated to determine fish condition and reproductive status. Moreover, a set of biomarkers including biotransformation enzymes, oxidative stress parameters, neurotoxicity and endocrine disruption markers was measured. The results allowed to determine biomarker variability due to fish gender or sampling season. For example, 7-ethoxyresorufin-O-deethylase activity, glutathione peroxidase as well as vitellogenin and spiggin exhibited strong gender differences. Conversely, lipoperoxidation and acethylcholinesterase activity were characterised by a lack of gender and seasonal variation, and can be considered as more robust parameters for a field application. The present work allowed to establish practical guideline for biomarker measurements in wild sticklebacks and to define a reference system which can be used to analyze variations in future monitoring studies.
