Picoplankton and virioplankton abundance and community structure in Pearl River Estuary and Daya Bay, South China

By using flow cytometry techniques, we investigated the abundance and composition of the heterotrophic prokaryotes, virioplankton and picophytoplankton community in the Pearl River Estuary and Daya Bay in the summer of 2012. We identified two subgroups of prokaryotes, high nucleic acid (HNA) and low nucleic acid (LNA), characterized by different nucleic acid contents. HNA abundance was significantly correlated with larger phytoplankton and Synechococcus (Syn) abundance, which suggested the important role of organic substrates released from primary producers on bacterial growth. Although LNA did not show any association with environmental variables, it was a vital component of the microbial community. In contrast to previous studies, the total virioplankton concentration had a poor relationship with nutrient availability. The positive relationship between large-sized phytoplankton abundance and the V–I population confirmed that V–I was a phytoplankton-infecting viral subgroup. Although the V–II group (bacteriophages) was dominant in the virioplankton community, it was not related with prokaryotic abundance, which indicated factors other than hosts controlling V–II abundance or the uncertainty of virus-host coupling. With respect to the picophytoplankton community, our results implied that river input exerted a strong limitation to Syn distribution in the estuary, while picoeukaryotes (Euk) were numerically less abundant and showed a quite different distribution pattern from that of Syn, and hence presented ecological properties distinct from Syn in our two studied areas.

Graphical abstractOur results identified two subgroups of prokaryotes, HNA and LNA (a), characterized by different nucleic acid contents. HNA abundance was significantly correlated with larger phytoplankton and Synechococcus (Syn) abundance, suggested that the important role of organic substrates released from primary producer on bacterial growth. We also observed a positive relationship between large-sized phytoplankton abundance and V–I population (b) which confirmed that V–I was a phytoplankton infecting viral subgroup. Although V–II group (bacteriophages) was dominated in the virioplankton community, it was not related with bacterial abundance, which indicated factors other than hosts controlling V–II abundance or the uncertainty of virus-host coupling.Figure optionsDownload full-size imageDownload as PowerPoint slide