Direct evidences on bacterial growth pattern regulating pyrene degradation pathway and genotypic dioxygenase expression

•Nutrient broth could enhance the whole degradation rate of pyrene.•Bacteria could achieve a higher degradation efficiency per cell in the PAH substrates.•Good correlations between bacterial degradation rate and 4,5-pyrenediol or nidA gene•Growth patterns regulated degradation pathway and dioxygenase expression.

Pyrene degradation by Mycobacterium sp. strain A1-PYR was investigated in the presence of nutrient broth, phenanthrene and fluoranthene, respectively. Fast bacterial growth in the nutrient broth considerably enhanced pyrene degradation rate, whereas degradation efficiency per cell was substantially decreased. The addition of nutrient broth could not alter the transcription levels of all dioxygenase genotypes. In the PAH-only substrates, bacterial growth completely relied on biological conversion of PAHs into the effective carbon sources, which led to a higher degradation efficiency of pyrene per cell than the case of nutrient broth. Significant correlations were only observed between nidA-related dioxygenase expression and pyrene degradation or bacterial growth. The highest pyrene degradation rate in the presence of phenanthrene was consistent with the highest transcription level of nidA and 4,5-pyrenediol as the sole initial metabolite. This study reveals that bacterial growth requirement can invigorate degradation of PAHs by regulating metabolic pathway and genotypic enzyme expression.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide