Spatial and temporal evolution of organic foulant layers on reverse osmosis membranes in wastewater reuse applications

•Sequential MBR and RO treatment of wastewater was studied at pilot scale.•Fouling of the RO membranes increased as the solids retention time increased.•Live cells and carbohydrates on the RO membrane became more aggregated with time.•RO membrane fouling increased with more carbohydrates in the membrane foulant layer.•The distribution of proteins in the foulant layer did not change over time or with SRT.

Advanced treatment to remove trace constituents and emerging contaminants is an important consideration for wastewater treatment for potable reuse, and reverse osmosis (RO) can be a suitable technology to provide the necessary level of treatment. However, membrane fouling by biological and organic matter is a concern. This research examined the development of the RO membrane fouling layer using a bench-scale membrane bioreactor operating at different solids retention times (SRTs), followed by a custom-designed RO test cell. The RO test cell contained stacked plates that sandwich five sheets of RO membrane material, which can be extracted for autopsy at separate times over the course of an experiment without disturbing the remaining membranes. The MBR-RO system was run continuously for 2 weeks at each SRT. The RO membranes were stained for live and dead cells, protein, and carbohydrate-like materials, and visualized using confocal laser scanning microscopy. Images of the stained foulant layers were obtained at different depths within the foulant layer at each time point for all SRT conditions. As the RO foulant layer developed, changes occurred in the distribution and morphology of the live cells and carbohydrates, but not the proteins. These trends were similar for all three SRT conditions tested. RO membrane fouling increased with increased MBR SRT, and the highest SRT had the highest ratios of live to dead cells and carbohydrate-like material to dead cells. The autopsied membranes were also analyzed for protein and carbohydrate content, and it was found that the carbohydrate concentration on the membranes after 14 days increased as the SRT increased.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (394 K)Download as PowerPoint slide