Effects of silver nanoparticles on wastewater biofilms

The goal of this research is to understand the potential antibacterial effect of silver nanoparticles (Ag-NPs) on biological wastewater treatment processes. It was found that original wastewater biofilms are highly tolerant to the Ag-NP treatment. With an application of 200 mg Ag/L Ag-NPs, the reduction of biofilm bacteria measured by heterotrophic plate counts was insignificant after 24 h. After the removal of loosely bound extracellular polymeric substances (EPS), the viability of wastewater biofilms was reduced when treated under the same conditions. By contrast, when treated as planktonic pure culture, bacteria isolated from the wastewater biofilms were highly vulnerable to Ag-NPs. With a similar initial cell density, most bacteria died within 1 h with the application of 1 mg Ag/L Ag-NPs. The results obtained here indicate that EPS and microbial community interactions in the biofilms play important roles in controlling the antimicrobial effects of Ag-NPs. In addition, slow growth rates may enhance the tolerance of certain bacteria to Ag-NPs. The effects of Ag-NPs on the entire microbial community in wastewater biofilms were analyzed using polymerase chain reaction–denaturing gradient gel electrophoresis, PCR–DGGE. The studies showed that the microbial susceptibility to Ag-NPs is different for each microorganism. For instance, Thiotrichales is more sensitive to Ag-NPs than other biofilm bacteria.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (107 K)Download as PowerPoint slideHighlights► Antibacterial effects of silver nanoparticles (Ag-NPs) on wastewater biofilms. ► Intact wastewater biofilms are highly tolerant to Ag-NPs. ► Biofilm bacteria, with loosely bound EPS removed, are more sensitive to Ag-NPs. ► Biofilm bacteria, treated as isolated pure culture, are much more sensitive to Ag-NPs. ► Susceptibility to Ag-NPs is different for each biofilm microorganism.