Cohesiveness and hydrodynamic properties of young drinking water biofilms

Drinking water biofilms are complex microbial systems mainly composed of clusters of different size and age. Atomic force microscopy (AFM) measurements were performed on 4, 8 and 12 weeks old biofilms in order to quantify the mechanical detachment shear stress of the clusters, to estimate the biofilm entanglement rate ξ. This AFM approach showed that the removal of the clusters occurred generally for mechanical shear stress of about 100 kPa only for clusters volumes greater than 200 μm3. This value appears 1000 times higher than hydrodynamic shear stress technically available meaning that the cleaning of pipe surfaces by water flushing remains always incomplete. To predict hydrodynamic detachment of biofilm clusters, a theoretical model has been developed regarding the averaging of elastic and viscous stresses in the cluster and by including the entanglement rate ξ. The results highlighted a slight increase of the detachment shear stress with age and also the dependence between the posting of clusters and their volume. Indeed, the experimental values of ξ allow predicting biofilm hydrodynamic detachment with same order of magnitude than was what reported in the literature. The apparent discrepancy between the mechanical and the hydrodynamic detachment is mainly due to the fact that AFM mechanical experiments are related to the clusters local properties whereas hydrodynamic measurements reflected the global properties of the whole biofilm.

► Biofilm mechanical behavior depend on cohesiveness strength profile determined by AFM. ► Mechanical removal of biofilm aggregates occurs layer by layer (stratified structures). ► Biofilm detachment shear stress weakly impacted by aging and the hydrodynamic formation conditions. ► Young drinking water biofilms behave like a viscoelastic solid with a low entanglement rate of about 6·10−4 to 10−3. ► Combination of mechanical measurements and ξ values allow biofilm hydrodynamic detachment prediction.