Dynamic approaches of mixed species biofilm formation using modern technologies

Bacteria and diatoms exist in sessile communities and develop as biofilm on all surfaces in aqueous environments. The interaction between these microorganisms in biofilm was investigated with a bacterial genus Pseudoalteromonas sp. (strain 3J6) and two benthic diatoms Amphora coffeaeformis and Cylindrotheca closterium. Each biofilm was grown for 22 days. Images from the confocal microscopy show a difference of adhesion between Pseudoalteromonas 3J6 and diatoms. Indeed, a stronger adhesion is found with C. closterium suggesting cohabitation between Pseudoalteromonas 3J6 and C. closterium compared at an adaptation for bacteria and A. coffeaeformis. The cellular attachment and the growth evolution in biofilm formation depend on each species of diatoms in the biofilm. Behaviour of microalgae in presence of bacteria demonstrates the complexity of the marine biofilm.

► One marine bacterium and two benthic marine diatoms were used for biofilm formation and determine their interaction. ► Characterization by CLSM and SEM revealed the architecture of biofilm changes. ► The cellular attachment and the growth evolution in biofilm formation depend on each species of diatoms in the biofilm. ► Behaviour of microalgae in presence of bacteria demonstrates the complexity of the marine biofilm.