Denitrification prevails over anammox in tropical mangrove sediments (Goa, India)

Denitrification, anammox (Anx) and di-nitrogen fixation were examined in two mangrove ecosystems- the anthropogenically influenced Divar and the relatively pristine Tuvem. Stratified sampling at 2 cm increments from 0 to 10 cm depth revealed denitrification as the main process of N2 production in mangrove sediments. At Divar, denitrification was ∼3 times higher than at Tuvem with maximum activity of 224.51 ± 6.63 nmol N2 g−1 h−1 at 0–2 cm. Denitrifying genes (nosZ) numbered up to 2 × 107 copies g−1 sediment and belonged to uncultured microorganisms clustering within Proteobacteria. Anammox was more prominent at deeper depths (8–10 cm) mainly in Divar with highest activity of 101.15 ± 87.73 nmol N2 g−1 h−1 which was 5 times higher than at Tuvem. Di-nitrogen fixation was detected only at Tuvem with a maximum of 12.47 ± 8.36 nmol N2 g−1 h−1. Thus, in these estuarine habitats prone to high nutrient input, N2-fixation is minimal and denitrification rather than Anx serves as an important mechanism for counteracting N loading.

► Denitrification, anammox and di-nitrogen fixation were examined in two mangrove ecosystems of Goa, India. ► In anthropogenically influenced Divar and relatively pristine Tuvem, denitrification and anammox co-occur to produce N2. ► But, at anthropogenically influenced Divar, anammox is restrained and denitrification becomes the main mechanism of N loss. ► Thus, with high nutrient input, N2-fixation is minimal and denitrification is the major pathway for countering N loading.