Acute toxicity of Roundup® herbicide to three life stages of the freshwater shrimp Caridina nilotica (Decapoda: Atyidae)

Glyphosate based herbicides, including Roundup®, are frequently used in the chemical control of weeds and invading alien plant species in South Africa. These herbicides ultimately get into water courses directly or indirectly through processes such as drifting, leaching, surface runoff and foliar spray of aquatic nuisance plants. Despite their widespread use, no water quality guideline exists to protect indigenous South African freshwater organisms from the toxic effects of these herbicides. The toxicity of the herbicide Roundup® was assessed using three different life stages of the freshwater shrimp Caridina nilotica, a prevalent species in South African freshwater ecosystems. Neonate (<7 days post hatching (dph)), juvenile (>7 dph and <20 dph) and adult (>40 dph) shrimps were exposed to varying concentrations (1.5–50 mg/L acid equivalence (a.e.)) of the herbicide in 48 and 96 h acute toxicity tests in order to determine the most sensitive life-stage. The results showed neonates to be more sensitive to Roundup® than both juveniles and adults with mean 96 h LC50 values of 2.5, 7.0 and 25.3 mg/L a.e. respectively. The estimated 96 h LC50 of neonates is much lower than the application rate (20–30 mg/L a.e.), although the application’s impact will depend on the dilution rate of the applied concentration in the environment. All three life-stages of unexposed animals exhibited active and coordinated movement but exposed shrimps were erratic and slow in their movements, with neonates showing most of these behavioral irregularities. This study shows that low levels of the herbicide Roundup® may adversely affect C. nilotica health and survival. Thus, the herbicide should be carefully managed to minimize any negative impact on non-target freshwater organisms.

► Toxicity of Roundup® was assessed using three life stages of Caridina nilotica. ► 48 and 96 h Acute tests were performed to determine the most sensitive life stage. ► Neonates were most sensitive to Roundup®, followed by juveniles and adults. ► Roundup® low levels may adversely affect C. nilotica health and survival.