| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4764850 | Current Opinion in Chemical Engineering | 2017 | 12 Pages |
Abstract
â¢Current status, challenges and outlook for CRISPR/Cas9 genome engineering in hPSCs.â¢Applications in investigating human biology, disease and therapy.â¢Novel applications of dCas9-based fusions.
Human pluripotent stem cells (hPSCs) hold great promise for modeling and recapitulation of human biological processes. Coupled with the advent of genome engineering tools, specifically the CRISPR-Cas9 systems, hPSCs have opened a multitude of possibilities in modeling human biology and creating novel cellular therapies. Here, we review the fundamentals of both technologies, and the wide-ranging present and future applications of genome engineering in hPSCs.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Udit Parekh, Marianna Yusupova, Prashant Mali,