A novel fluorescent probe based on β-C-glycoside for quantification of bovine serum albumin

Two novel C-glycoside-coumarin fluorescent dyes are reported and their UV/Vis, fluorescence spectra and fluorescence-response to bovine serum albumin (BSA) are investigated and discussed. The fluorescence emission is red-shifted and quenched with the increase in polarity of solvents. Moreover, the fluorescence intensity of the dyes increased upon the addition of BSA in pH 7.4 phosphate buffer solution. An excellent linear relationship (R2 = 0.99495) for compound 1 was obtained between fluorescence intensity and bovine serum albumin concentration. The method show a good selectivity and sensitivity for BSA over few proteins, other ions and amino acids, which was suggested as high selective and sensitive fluorescent reagents for quantification of BSA in biological science.