Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4766203 | Dyes and Pigments | 2017 | 11 Pages |
Abstract
Two new Ru(II) complexes [Ru(dtzp)(dppz)Cl]+1 and [Ru(dtzp)(dppz)CH3CN]2+2 (dtzp = 2,6-di(thiazo1-2-yl)pyridine; dppz = dipyrido[3,2-a:2â²,3â²-c]phenazine) have been synthesized and evaluated as photodynamic anticancer agents. The results of the spectra titration, thermal denaturation and electrophoresis experiments suggest that both complexes could intercalatively bind to DNA and photocleave DNA efficiently by ROS generation and photoinduced electron transfer. When incubated under visible light (470 nm), complex 1 and 2 generate great photocytoxicity towards Hela cells in both 2D cancer cell monolayer and 3D MCTS cancer models, and a much greater photocytotoxicity was observed for complex 1, which may be associate with its' larger cellular uptake efficiency and stronger absorption at 470 nm. Flow cytometry analysis and immunofluorescence assay revealed that complex 1 inhibited Hela cell proliferation through G2M phase cycle arrest and cell apoptosis and could generate great photodamage to chromatin DNA. Complex 1 may be a prominent PDT candidate used for treating cervical carcinoma.
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Authors
Hui-juan Yu, Jiang-ping Liu, Zhi-feng Hao, Jun He, Ming Sun, Sheng Hu, Lin Yu, Hui Chao,