A 3D bio-platform constructed by glucose oxidase adsorbed on Au nanoparticles assembled polyaniline nanowires to sensitively detect glucose by electrochemiluminescence

•Well-defined PANi nanowires network was formed on electrode by electro-polymerization.•Smart 3D bio-platform of GOD/AuNPs/PANi was easily constructed by self-assembling.•GOD/AuNPs/PANi modified sensor exhibits remarkable performance for glucose detection by ECL.

In this paper, a high-performance three dimensional (3D) bio-platform of glucose oxidase (GOD) adsorbed on Au nanoparticles (AuNPs) assembled polyaniline (PANi) nanowires network (GOD/AuNPs/PANi) was constructed to sensitively detect glucose by electrochemiluminescent (ECL) technology for the first time. Due to the unique structure of porous network, excellent conductivity and good biocompatibility for enzyme, the bio-platform enable highly strong the ECL emission, which make the proposed biosensor achieves a wide linear range of 0.1–100 μM (R2 = 0.992), extremely low detection limit of 0.05 μM (S/N = 3), excellent selectivity and good stability in glucose detection. When the proposed biosensor was used in serum samples determination, a satisfactory result also obtained.