Pt modified carbon fiber microelectrode for electrochemically catalytic reduction of hydrogen peroxide and its application in living cell H2O2 detection

In this contribution, a new carbon fiber microelectrode (CFME) for detection of hydrogen peroxide (H2O2) is constructed with a rapid and low-cost method based on the electrochemical etching technique and the electro-deposition of platinum (Pt) nanoparticles, and the potential application in electrochemical bioanalysis has been explored. It is observed that when the CFME modified in 5 mmol/L chloroplatinic acid with 0.5 mol/L H2SO4 used as supporting electrolyte, the as-prepared Pt modified carbon fiber microelectrode (Pt/CFME) demonstrates an excellent electrochemically catalytic activity, which exhibited a wide linear in relevant detection for the reduction of H2O2, ranging from 0.044 to 12.30 mmol/L with the limit of 44 μmol/L. Moreover, our results illustrate that the Pt/CFME electrode can be readily utilized in scanning electrochemical microscope (SECM) studies to detect H2O2 release from human glioblastoma cells U87 when stimulated with ascorbic acid (AA).