Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4908182 | Journal of Electroanalytical Chemistry | 2016 | 6 Pages |
Abstract
We have developed a fast, highly sensitive and low-cost biosensing system for the detection of clenbuterol (CLB), using a homemade mobile electrochemical device with an electric field-driven acceleration strategy. This system consists of an embedded circuit in smartphone for signal processing and a screen-printed carbon electrode (SPCE) modified with multi-walled carbon nanotubes (MWNTs) and goat anti mouse-immunoglobulin G (IgG) sensing layer (MWNTs-I-layer). CLB monoclonal antibody was assembled through its binding to the surface-confined antibody. Such modified electrodes were used for rapid and sensitive amperometric immunosensing detection of CLB. Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody. By using this mobile system, we could detect CLB ranging from 0.3 ng â
 mLâ 1 to 100 ng â
 mLâ 1 with the detection limit of 0.076 ng â
 mLâ 1. The whole competitive-type detection process was finished within 6 min. We expect this device can meet the requirements for field detection of various food security-related species.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Yanzhi Dou, Zhineng Jiang, Wangping Deng, Jing Su, Shixing Chen, Haiyun Song, Ali Aldalbahi, Xiaolei Zuo, Shiping Song, Jiye Shi, Chunhai Fan,