Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4908550 | Journal of Environmental Chemical Engineering | 2016 | 7 Pages |
Abstract
Enzyme catalyzed reactions have been considered to be friendly and thus “green” for the environment. The two main requisites for developing an enzyme based amperometric sensor for biomedical as well as industrial applications are a robust support for the enzyme and an efficient conduit for electron conduction. Keeping in view the aforementioned requirements, we designed an electrode using selectively functionalized multi-walled carbon nanotube dispersed in polyvinyl alcohol (PVA-MWCNT) matrix. Glucose oxidase (GOx) enzyme was immobilized on this electrode and glucose concentration was monitored to determine the efficiency of the (PVA-MWCNT) membrane coated glassy carbon electrode. The load of GOx immobilized on the membrane achieved was as high as 3.98Â ÃÂ 10â9Â mol/cm2. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) was used as an analytical tool to show immobilized GOx enzyme. The electron transfer rate constant (ks) of the modified glassy carbon electrode obtained by cyclic voltammetry measurements was 1.58Â sâ1 which shows a rapid electron transfer process and high sensitivity of 8.67Â mAÂ mMâ1Â cmâ2 indicating a better response as compared to the values reported till now. This is the first report of such high load of enzyme on functionalized PVA which contributes to the enhanced electron conduction leading to the detection of glucose over a wider linear range of 0.1-20.0Â mM. Detection limit of the electrode for glucose is 0.15Â mM.
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Chemical Engineering (General)
Authors
S. Gupta, C. Ratna Prabha, C.N. Murthy,