Biofabrication of copper oxide nanoparticles using Andean blackberry (Rubus glaucus Benth.) fruit and leaf

Biofabrication of copper oxide nanoparticles (CuO-NPs) of a desired size remains a significant challenge. In this report, CuO-NPs were fabricated by treating 10 mM copper nitrate with Andean blackberry fruit (ABF) and leaf (ABL); and evaluated its antioxidant activity. As-prepared NPs characterization were determined by UV-visible spectrophotometry, Dynamic Light Scattering (DLS), transmission electron microscopy (TEM) with selected area electron diffraction (SAED) and X-ray diffraction (XRD) analysis. UV-visible spectroscopy showed an electronic excitonic transition at 250-255 nm clearly reveals the formation of ABF and ABL CuO-NPs. DLS analysis demonstrated mean diameter of ABF CuO-NPs (43.3 nm) smaller than ABL CuO-NPs (52.5 nm). TEM with SAED confirmed the CuO-NPs are spherical and of partial crystalline nature. Furthermore, the antioxidant efficacy of ABF CuO-NPs showed 89.02%, 1 mM whereas ABL CuO-NPs 75.92%, 1 mM against 1,1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that green CuO-NPs could be used effectively in future biomedical concerns.