Synthesis, spectral characterization and biological evaluation of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(-2-pyridyl)-4-pyrazolines via Michael addition

A series of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(2-pyridyl)-4-pyrazolines derivatives have been synthesized using sodium acetate as a catalyst. The spectral characterization and structure of 1-thiocarbamoyl-3-phenyl-5-hydroxy-5-(2-pyridyl)-4-pyrazolines are reported. Spectral techniques employed include 1H NMR, 13C NMR, 1H-1H COSY, HSQC, HMBC, D2O exchange, Mass and IR. Compounds 12-22 exhibited potent antibacterial activity against Salmonella typhi and Pseudomonas aeruginosa whereas the same set of compounds exerted potent antifungal activity against Aspergillus niger and Aspergillus fumigatus.