Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4926054 | Renewable Energy | 2017 | 39 Pages |
Abstract
Eucalyptus has been previously suggested as a potential substrate for acetone-butanol-ethanol (ABE) production without nutrient supplementation; however, incomplete sugar consumption has prevented improvement of ABE production. Cellulase loading with 35 FPU gâ1 was first optimized in terms of high hydrolysis efficiency (95%). However, only 0.43 g Lâ1 ABE production and 3.44% glucose consumption rate were achieved. To improve ABE production from eucalyptus hydrolysate, supplementation of 6 nutrients in common tryptone-yeast extract (TY) medium were investigated by statistical approaches. Three nutrients including yeast extract, tryptone, and FeSO4·7H2O were screened as significant nutrients for ABE production. Subsequently, use of a modified TY medium (MTY medium: yeast extract 3.04 g Lâ1, tryptone 7.64 g Lâ1, FeSO4·7H2O 15.3 mg Lâ1), which was subsequently predicted by Plackett-Burman and Box-Behnken designs to stimulate ABE production, resulted in ca. 40-fold increase in ABE concentration (16.9 g Lâ1) and a glucose consumption rate of 100%. We first examined previously uninvestigated nutrition combinations using Plackett-Burman and Box-Behnken designs for high ABE production from eucalyptus hydrolysate. This study shows that statistical method would be a powerful tool for the optimization and enhancement of ABE production from eucalyptus hydrolysate.
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Authors
Jin Zheng, Yukihiro Tashiro, Tao Zhao, Qunhui Wang, Kenji Sakai, Kenji Sonomoto,