Electroenzymatic process to overcome enzyme instabilities

•H2O2 can be produced using a gas diffusion cathode depending on the applied current.•ttn of up to 1,150,000 was achieved for CPO using an electroenzymatic system.•CPO is more stable than assumed.•Novel dimensionless parameter for process design is introduced.•Space time yields of up to 52 g L− 1 d− 1 can be reached.

The versatile enzyme chloroperoxidase was used in a reaction system, based on a gas diffusion electrode, for enzymatic chlorinations. Due to an adjusted and continuous electro-generation of the co-substrate hydrogen peroxide a ttn up to 1,150,000 for the CPO was achieved. Space time yields were dependent on the electrochemically produced H2O2 and reached up to 52 g L− 1 d− 1. The ratio of hydrogen peroxide production per added enzyme unit can be used as a dimensionless parameter for process characterization and a knowledge based process design.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide