ProtocolsSuperficial physicochemical properties of polyurethane biomaterials as osteogenic regulators in human mesenchymal stem cells fates

•Biodegradable and biocompatible polyester/ether based polyurethanes were synthesized.•Surface and bulk properties of prepared 2D membranes were evaluated.•The hMSCs showed greater osteogenesis expression in PUs with more roughness.•The hMSCs showed greater osteogenesis expression in PUs with more stiffness.•Surface parameters along with other cues have a synergistic effect on hMSCs fates.

All of the cells' interactions are done through their surfaces. Evaluation of surface physicochemical scaffolds along with other factors is important and determines the fate of stem cells. In this work, biodegradable and biocompatible polyester/polyether based polyurethanes (PUs) were synthesized by polycaprolactone diol (PCL) and poly (tetra methylene ether) glycol (PTMEG) as the soft segment. To assess better the impact of surface parameters such as stiffness and roughness effects on osteogenic differentiation of the human mesenchymal stem cell (hMSC), the dimension effect of substrates was eliminated and two-dimensional membranes were produced by synthesized polyurethane. Surface and bulk properties of prepared 2D membranes such as surface chemistry, roughness, stiffness and tensile behavior were evaluated by Attenuated total reflectance Fourier transform infrared (ATR-FTIR), atomic force microscopy (AFM) and tensile behavior. The prepared 2D PU films had suitable hydrophilicity, biodegradability, water absorption, surface roughness and bulk strength. The hMSCs showed greater osteogenesis expression in PU substrates with more roughness and stiffness than others. The results demonstrated that surface parameters along with other differentiation cues have a synergistic effect on stem cells fates.

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