Silk biomaterials functionalized with recombinant domain V of human perlecan modulate endothelial cell and platelet interactions for vascular applications

•Perlecan is an essential vascular proteoglycan.•C-terminal domain V of human perlecan expressed recombinantly as a proteoglycan.•Silk biomaterials functionalised with recombinant domain V.•Immobilization technique and glycosaminoglycan chains affected domain V orientation.•Domain V orientation modulated endothelial cell and platelet interactions.