| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4996922 | Bioresource Technology | 2017 | 5 Pages |
Abstract
Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO3. One of the isolate showed resistance to NaHCO3 (100Â mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with proteomic analysis by LC-MS/MS. In proteomic analysis 96 proteins were identified out of these 6 protein involved in carbon dioxide fixation, 11 in fatty acid metabolism, indicating the carbon dioxide fixing potency of bacterium along with production of biofuel. GC-MS analysis revealed that hydrocarbons and FAMEs produced by bacteria within the range of C13-C24 and C11-C19 respectively. Presence of 59% saturated and 41% unsaturated organic compounds, make it a better fuel composition.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Manish Kumar, Raj Morya, Edgard Gnansounou, Christian Larroche, Indu Shekhar Thakur,