Quantitative characterization of enzyme adsorption and hydrolytic performance for ultrafine grinding pretreated corn stover

•Ultrafine grinding is efficient in changing the microstructure properties.•The quantitative analysis of enzyme adsorption and hydrolysis were presented.•The enzyme adsorption was exponential to hydrolytic production for UGCS.•The binding enzyme proportion was inversely proportional to enzyme consumption.

Quantitative analysis of enzyme adsorption and hydrolysis were performed for sieve-based grinding corn stover (SGCS) and ultrafine grinding corn stover (UGCS)1 with different enzyme consumptions. The UGCS presented significantly higher enzyme adsorption quantity (5.15 mg/g for UGCS, 1.33 mg/g for SGCS), higher glucose yield (49.75% for UGCS, 28.75% for SGCS) under 20 FPU/g and higher binding enzyme proportion (41.32% for UGCS, 10.64% for SGCS under 5 FPU/g) which can be attributed to the more accessible microstructure properties. The relationship between enzyme adsorption and hydrolytic production was directly proportional for SGCS (GY1 = 21.04 × AQ1 + 1.86 (R2 = 0.95)) while was exponential for UGCS (GY2 = 49.42 × (1 − e−0.57×AQ2) (R2 = 0.99)),2 indicating that overmuch enzyme consumption was not advisable for UGCS at economical aspect.

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