Enhancement of microbial density and methane production in advanced anaerobic digestion of secondary sewage sludge by continuous removal of ammonia

•NH3 inhibition was mitigated by continuous NH4+ removal using ion-exchangers.•CH4 yields improved by 54% when NH3 reduced from 630 to 92 mg/L.•Population density of MSC and MBT increased by over 6 and 5 times.•Total bacterial density increased by over 2 times facilitated by ammonia reduction.•Enhanced carbohydrates and proteins hydrolysis was achieved with ammonia removal.

Ammonia inhibition mitigation in anaerobic digestion of high solids content of thermally hydrolysed secondary sewage sludge by the NH4+ affinitive clinoptilolite and a strong acid type ion-exchange resin S957 was investigated. Continuous NH4+-N removal was achieved through ion-exchanging at both temperatures with average removals of 50 and 70% for the clinoptilolite and resin dosed reactors, respectively. Approximate 0.2-0.5 unit of pH reduction was also observed in the dosed reactors. The synergy of NH4+-N removal and pH reduction exponentially decreased free NH3 concentration, from 600 to 90 mg/L at 43 °C, which mitigated ammonia inhibition and improved methane yields by approximately 54%. Microbial community profiling suggested that facilitated by ammonia removal, the improvement in methane production was mainly achieved through the doubling in bacterial density and a 6-fold increase in population of the Methanosarcinaceae family, which in turn improved the degradation of residual volatile fatty acids, proteins and carbohydrates.

Graphical abstractDownload high-res image (104KB)Download full-size image