Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4997616 | Bioresource Technology | 2017 | 32 Pages |
Abstract
The aim of this work was to study the inhibition and deactivation of commercial enzyme cocktail (Cellic® Htec2) in the presence of [bmim][Cl] ionic liquid employing model cellulosic substrate, carboxymethyl cellulose (CMC). It turned out from the experiments - relying on enzyme kinetics approach - that [bmim][Cl] could act as a competitive inhibitor. Furthermore, depending on the process conditions i.e. contact of enzyme solution with high concentration [bmim][Cl], severe biocatalyst inactivation should be also taken into account as a potential risk during the enzymatic cellulose hydrolysis even in as short process times as few minutes.
Related Topics
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Process Chemistry and Technology
Authors
Nándor Nemestóthy, Gábor Megyeri, Péter Bakonyi, Patrik Lakatos, László Koók, Milan Polakovic, László Gubicza, Katalin Bélafi-Bakó,