Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5002910 | IFAC-PapersOnLine | 2016 | 6 Pages |
Abstract
Real-time automatic regulation of gene expression is a key technology for synthetic biology enabling, for example, synthetic circuit components to operate in an optimal range. We show that it is possible to regulate the expression of a reporter protein from the tetracycline-inducible promoter in a population of mammalian cells using principles from automatic control engineering. We demonstrate that the performance of the control experiments improves by moving from a discrete to a continuous actuation. Our automated control platform is an innovative tool to enable dose-response studies of small molecules and investigation of gene dosage effects in disease.
Related Topics
Physical Sciences and Engineering
Engineering
Computational Mechanics
Authors
Lorena Postiglione, Marco Santorelli, Barbara Tumaini, Diego di Bernardo,