Metabolic fluxes in recombinant Streptomyces lividans analyzed with 13C-based metabolic flux analysis

The cellulase A producing strain shows an increased secretion of organic acids, while growth is less efficient. Intracellularly, an increase through the pentose phosphate pathway and the citric acid cycle is observed, which alters the redox potential. Production of NADH and NADPH is higher in the CelA-producing strain, although the need is expected to be lower.