Article ID Journal Published Year Pages File Type
5008660 Sensors and Actuators B: Chemical 2018 6 Pages PDF
Abstract

Slab gel electrophoresis (SGE) is very common tool for DNA, RNA and protein analysis, but it is tedious, labor-intensive, skill-dependent, and relatively slow. Herein, we developed a biochip based SGE system, which can resolve the DNA fragments and record their separation process. By electrophoresis of 50 bp DNA ladder, we found that the 16 DNA fragments were effectively resolved within 14 min. In order to validate its feasibility and practicability, we take periodontal pathogens (e.g., Porphyromonas gingivalis (P.g), Tannerela forsythia (T.f), and Treponema denticola (T.d)) as an example by separating their polymerase chain reaction products. Experiments demonstrated that P.g, T.d and T.f were diagnosed within 12 min, and the electrophoresis of P.g showed that the detection limit of this system was about 6.4 ng/μl. Such a low cost system is easy to operate, and can effectively improve SGE in the biological experiment, especially for the labs in the third world countries.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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