Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5030756 | Biosensors and Bioelectronics | 2018 | 19 Pages |
Abstract
In this work, a novel ultrasensitive electrochemical biosensor was developed for the detection of K562 cell by a signal amplification strategy based on multiple layer CdS QDs functionalized polystyrene microspheres(PS) as bioprobe and graphene oxide(GO) -polyaniline(PANI) composite as modified materials of capture electrode. Due to electrostatic force of different charge, CdS QDs were decorated on the surface of PS by PDDA (poly(diallyldimethyl-ammonium chloride)) through a layer-by-layer(LBL) assemble technology, in which the structure of multiple layer CdS QDs increased the detection signal intensity. Moreover, GO-PANI composite not only enhanced the electron transfer rate, but also increased tumor cells load ratio. The resulting electrochemical biosensor was used to detect K562 cells with a lower detection limit of 3 cells mLâ1 (S/N = 3) and a wider linear range from 10 to 1.0 à 107 cells mLâ1. This sensor was also used for mannosyl groups on HeLa cells and Hct116 cells, which showed high specificity and sensitivity. This signal amplification strategy would provide a novel approach for detection, diagnosis and treatment for tumor cells.
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Authors
Jidong Wang, Xiaoyu Wang, Hengshan Tang, Zehua Gao, Shengquan He, Jian Li, Shumin Han,