Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5030759 | Biosensors and Bioelectronics | 2018 | 21 Pages |
Abstract
In this work, a novel non-enzymatic superoxide anion (O2
- â) sensor was constructed based on Ag nanoparticles (NPs) / poly (amidoamine) (PAMAM) dendrimers and used to investigate the dynamic process of O2
- â release from living cells. The AgNPs/PAMAM nanohybrids were characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The fabricated electrode exhibited excellent catalytic activity toward the reaction of O2
- â with a super low detection limit (LOD) of 2.530 Ã 10-13Â M (S/N = 3) and wide linear range of 8 orders of magnitude. It could fulfill the requirement of real-time measurement O2
- â released from living cells. Furthermore, zymosan was chosen as the stimulant to induce O2
- â generation from cancer cells (rat adrenal medulla pheochromocytoma cell (PC12)). The electrochemical experiment results indicated that the levels of intracellular O2
- â depended on the amount of Zymosan. A large amount of O2
- â generated in the living cells by added heavy stimulant could damage cells seriously. More importantly, a vitro simulation experiment confirmed the role of superoxide dismutase (SOD) for the first time because it could maintain the O2
- â concentration at a normal physiological range. These findings are of great significance for evaluating the metabolic processes of O2
- â in the biological system, and this work has the tremendous potential application in clinical diagnostics to assess oxidative stress.
- â) sensor was constructed based on Ag nanoparticles (NPs) / poly (amidoamine) (PAMAM) dendrimers and used to investigate the dynamic process of O2
- â release from living cells. The AgNPs/PAMAM nanohybrids were characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The fabricated electrode exhibited excellent catalytic activity toward the reaction of O2
- â with a super low detection limit (LOD) of 2.530 Ã 10-13Â M (S/N = 3) and wide linear range of 8 orders of magnitude. It could fulfill the requirement of real-time measurement O2
- â released from living cells. Furthermore, zymosan was chosen as the stimulant to induce O2
- â generation from cancer cells (rat adrenal medulla pheochromocytoma cell (PC12)). The electrochemical experiment results indicated that the levels of intracellular O2
- â depended on the amount of Zymosan. A large amount of O2
- â generated in the living cells by added heavy stimulant could damage cells seriously. More importantly, a vitro simulation experiment confirmed the role of superoxide dismutase (SOD) for the first time because it could maintain the O2
- â concentration at a normal physiological range. These findings are of great significance for evaluating the metabolic processes of O2
- â in the biological system, and this work has the tremendous potential application in clinical diagnostics to assess oxidative stress.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hongwei Wei, Tianyi Shang, Tiaodi Wu, Guoan Liu, Lan Ding, Xiuhui Liu,