Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5030760 | Biosensors and Bioelectronics | 2018 | 7 Pages |
Abstract
In this paper, a trimetallic Pd@Au@Pt nanocomposites platform on -COOH terminated reduced graphene oxide (COOH-rGO) was newly developed for sensing carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) biomarkers. Trimetallic electro-catalytic surfaces were prepared by the electrodeposition of noble metals (Pd@Au@Pt) nanoparticles on COOH- rGO. After EDC/NHS treatment, the anti-CEA and anti-PSA were immobilized separately on two different platforms. Under optimized conditions, the platforms were analyzed by cyclic voltammetry and differential pulse voltammetry (DPV). The platform shows good electro catalytic activity, high sensitivity, and acceptable stability for sensing CEA and PSA biomarkers. For CEA, we obtained sensitivity of 0.099 ± 0.007 µA ngâ1 ml, wide linear range from 12 pg mlâ1 to 85 ng mlâ1 and a limit of detection (LOD) of 8 pg mlâ1, while for PSA sensitivity is 0.267 ± 0.02 µA ng â1 ml, wide linear range from 3 pg mlâ1 to 60 ng mlâ1 and LOD of 2 pg mlâ1. The validation of the platform was observed through standard addition method. Thus, the sensing platform could be used for the point of care detection of CEA and PSA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S.C. Barman, M.F. Hossain, Hyosang Yoon, Jae Y. Park,