Trimetallic Pd@Au@Pt nanocomposites platform on -COOH terminated reduced graphene oxide for highly sensitive CEA and PSA biomarkers detection

In this paper, a trimetallic Pd@Au@Pt nanocomposites platform on -COOH terminated reduced graphene oxide (COOH-rGO) was newly developed for sensing carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) biomarkers. Trimetallic electro-catalytic surfaces were prepared by the electrodeposition of noble metals (Pd@Au@Pt) nanoparticles on COOH- rGO. After EDC/NHS treatment, the anti-CEA and anti-PSA were immobilized separately on two different platforms. Under optimized conditions, the platforms were analyzed by cyclic voltammetry and differential pulse voltammetry (DPV). The platform shows good electro catalytic activity, high sensitivity, and acceptable stability for sensing CEA and PSA biomarkers. For CEA, we obtained sensitivity of 0.099 ± 0.007 µA ng−1 ml, wide linear range from 12 pg ml−1 to 85 ng ml−1 and a limit of detection (LOD) of 8 pg ml−1, while for PSA sensitivity is 0.267 ± 0.02 µA ng −1 ml, wide linear range from 3 pg ml−1 to 60 ng ml−1 and LOD of 2 pg ml−1. The validation of the platform was observed through standard addition method. Thus, the sensing platform could be used for the point of care detection of CEA and PSA.